Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Comparative performance of four LIT modes, Luminex, and ELISA

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Incubation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Schematic diagrams illustrating the methodologies used in Luminex® Assay ( a ) and ELISA ( b ). Figure was created with MedPeer (medpeer.cn). c Dose dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and Luminex (red). d Dose dependent median fluorescence intensity and absorbance of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and ELISA (red). Data presented as mean ± SD from three replicates. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Fluorescence, Standard Deviation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: a Dose-dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at various concentrations compared between Standard LIT assays with (red) and without (black) added serum. b Comparison of LODs for IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF with (pink) and without (blue) serum. c Spike recoveries for cytokines at high, medium, and low analyte concentrations. Linear regression plots comparing serum concentrations of IL-6 ( d ) and IL-8 ( e ) as determined by Luminex and LIT. Linear regression plots for IL-6 ( f ) and IL-8 ( g ) serum concentrations, determined by ELISA and LIT. Linear regression plots for IL-6 ( h ) and IL-8 ( i ) serum concentrations, determined by chemiluminescence and LIT. Data are presented as mean ± SD; n = 3 repeated tests. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Fluorescence, Comparison, Luminex, Enzyme-linked Immunosorbent Assay, Standard Deviation